BD PrepStain™
BD PrepStain™ Cytology Smear Preparation and Staining System – LifeMed Holding
The BD PrepStain System is a system for the automated preparation of thin layer cytology smears. With a simple cytological examination, cell pathology can be detected before the onset of a malignant stage.
The technological feature of the presented system is that the test material is taken into a special storage solution, which ensures its safety without destruction and loss of cells. At the same time, all cellular material is preserved, the cells do not change their morphological and immunocytochemical properties.
A very important advantage of the preparations of the BD PrepStain system is the possibility of their use for molecular biological studies, such as immunocytochemical detection of microorganisms in cervical smears.
Immunocytochemical method in thin-layer preparations can detect human papillomavirus, the detection of which is of great importance in cancer screening.
BD PrepStain is a universal system that can be used both for the preparation of cytological preparations in gynecology and for the study of other biological material.
The BD PrepStain System consists of several instruments with accessories and associated consumables.To obtain a full-fledged material from the cervix using BD PrepStain technology, a special tool is used to take the material – a plastic cervical brush.
The retaining liquid “preserves” the collected cellular material, prevents cell damage, avoids bacterial contamination, and allows cells to be delivered to the laboratory under optimal conditions. In addition, the storage liquid is resistant to temperature fluctuations, the material placed in the storage liquid can be stored for 1 month at room temperature and 6 months at +2°C to +8°C, used for genetic detection for oncogenicity (PCR studies for HPV or for control research).
After taking the material, the removable brush-tip is immersed directly in the retaining liquid.
The retaining liquid “preserves” the collected cellular material, prevents cell damage, avoids bacterial contamination, and allows cells to be delivered to the laboratory under optimal conditions. In addition, the storage liquid is resistant to temperature fluctuations, the material placed in the storage liquid can be stored for 1 month at room temperature and 6 months at +2°C to +8°C, used for genetic detection for oncogenicity (PCR studies for HPV or for control research).
Further, the procedure for preparing a cytological preparation includes obtaining a homogeneous suspension using a shaker. The tube shaking device is intended for shaking the cells from the removable brush-tip. This step is particularly necessary for the suspension of mucus-producing endocervical cells. Due to mucus, a significant proportion of these cells do not end up on the glass slide when using conventional preparation methods. Capable of processing up to 25 tubes.
The centrifuge is used to prepare a concentrated cell pellet, which is then processed by the PrepStain device.
Hettich Rotina 380
modern centrifuge with high safety performance, simple and easy to operate. Features smooth running and low noise. Excellent user performance is complemented by a wide range of accessories, which makes this centrifuge indispensable in almost any laboratory.
The centrifuge is intended for centrifugation of samples.
12-place centrifuge tube holders – 4 pcs
The PrepMate is designed for automatic preparation of cell suspension. They will perform the initial cell suspension enrichment process (consisting of purifying the suspension of red blood cells, detritus, and inflammatory elements): mix the sample and remove it from the preservative tube, then apply the sample to the dense reagent in the centrifuge tube.
The number of samples per run, which allows the automated process of the device to be processed from 1 to 12 samples per run.
Processing time for 12 samples – 3 minutes, 48 samples – 12 minutes.
Availability of a cork piercing process that allows mixing and application of the sample without removing the cork to reduce sample contamination.
The PrepStain device transfers cell samples from a centrifuge tube to a sedimentation chamber placed on a microscope slide. The PrepStain then automatically stains and rinses each slide.
The PrepStain system performs automated preparation of a micropreparation and step-by-step staining of a thin-layer preparation of cytological material on a glass slide.
The possibility of automating the stages of the process of preparing a micropreparation, which includes the preservation of cells, their randomization, enrichment with diagnostic material, pipetting, sedimentation, and staining.
The PrepStain cytology preparation and staining instrument allows the slide to be used for routine cytology screening and Bethesda categorization (classification of cervical-vaginal cytology diagnoses).
The presence of an adapter for a serial asynchronous RS232 interface, a hard disk with at least 10 MB of free space, a 3.5-inch floppy drive, at least 4 MB of RAM, IBM PC 386 or higher.
The monitor for the control computer is liquid crystal.
Keyboard for the control computer.
The #1 vacuum pump is designed to connect to the Prepstain aspirator block, which is connected to the pump via a vacuum waste container where the aspirated fluid is collected.
Vacuum pump #2 is connected to the Prepstain’s four-position manipulator. Intended for aspiration of liquid from sedimentation chambers on glass slides.
Starter kit for starting the system, consisting of: flushing liquid – plastic canister 3.6 l; preservative liquid BDSurePathTM – plastic canister 3.6l; dense reagent – a bottle of 480 ml; combined dye EA/OG – bottle 480 ml; dye hematoxylin 0.75 – bottle 480 ml; syringe-pipettes – 96 pcs.; settling chambers – 96 pcs., centrifuge tubes – 96 pcs.; slides BDSurePathTM PreCoat – 120 pcs.; disposable liquid transfer tips per pack — 96 pcs.; aspirator tips per pack – 96 pcs., TRIS powder for buffer solution preparation – 2 packs, dispenser and tubes; bottle and caps (5 pcs.).
Delivery set providing the process of preparation of micropreparations:
1. Device for preparing cytological preparations PrepMate – 1 pc.
2. Shaking device – 1 pc.
3. Device for staining cytological preparations PrepStain – 1 pc.
4. Monitor for the control computer of the BD PrepStain device – 1 pc.
5. Control unit with installed software for PrepStain painting machine – 1 pc.
6. Keyboard for the control computer of the BD PrepStain device – 1 pc.
7. Vacuum pump – 2 pieces
8. Starter kit to start the system – 1 set
9. Centrifuge Hettich Rotina 380 – 1 pc.
10. Uninterruptible power supply unit – 2 pcs.